1984年開(kāi)始,Case Gould就發(fā)表了一篇關(guān)于內毒素對細胞培養效應的綜述1。本文締一生物/締一生物為您分析內毒素對體外細胞生長(cháng)和功能的效應。
在這些效應中,包括刺激白細胞產(chǎn)生組織因子,激活小鼠巨噬細胞,抑制小鼠紅細胞集落形成(極低濃度即有該效應,小于1ng/ml)。那之后,許多文章發(fā)表,報道內毒素的各種效應,部分見(jiàn)下面的表格。
針對內毒素的刺激,免疫細胞的表現更為明顯。巨噬細胞和單核細胞已知會(huì )產(chǎn)生和釋放各種細胞因子,包括腫瘤壞死因子和白細胞介素等。Morris等人發(fā)現,馬腹腔巨噬細胞暴露在低至0.5ng/ml內毒素中6個(gè)小時(shí),也可引發(fā)顯著(zhù)的白細胞介素6產(chǎn)生2。Mattern顯示,100ng內毒素刺激人T細胞增生,產(chǎn)生淋巴因子3。
因此,選用盡可能低的內毒素的胎牛血清,顯然是優(yōu)上之選。
參考文獻
1. Case Gould, et al. Endotoxin in Vertebrate Cell Culture: Its Measurement and Significance. In Uses and Standardization of Vertebrate Cell Lines,(Tissue Culture Association, Gaithersburg, MD,1984),125-136.
2. Morris, D.D.,et al. Endotoxin-induced production of interleukin6 by equine peritoneal macrophages In Vitro. Am.J.Vet.Res.1992,53:1298-1301
3. Mattern, T., et al. Endotoxin and lipid A stimulate proliferation of human T cells in the presence of autologous monocytes. J.Immunol.1994,153:2996-3004
4. Ronald E.Unger, et al. Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay. Biomaterials. 2014,35(10):3180-3187
5. Rinehart J and Keville L. Effects of endotoxin on proliferation of human hematopoietic cell precursors. Cytotechnology (1997) 42:153-159.
6. Sivasubramaniyan K., et al. Endotoxin-induced silencing of mesoderm induction and functional differentiation: Role of HMGB1 in pluripotency and infection.Regenerative Medicine, 2008,3(1):23-31
7. Gorbet, M.B.; Sefton, M.V. Endotoxin: The uninvited guest. Biomaterials 2005, 26, 6811–6817,
綜上所述,您是不是已經(jīng)對內毒素對體外細胞生長(cháng)和功能的效應,有所了解。如果還有其他疑問(wèn),請咨詢(xún)締一生物/締一生物資深專(zhuān)家:。